The GenoTypeMycobacterium AS (Additional Species) and GenoTypeMycobacterium CM (Common Mycobacteria) is developed by Hain Lifescience for the identification of clinically relevant mycobacteria from solid and liquid cultures. This kits can be purchased separately although both tests have the same starting material and the hybridization can be performed with the same PCR product.

Advantages of Using GenoType Mycobacterium CM/AS:

  • Can be performed from solid or liquid culture samples.
  • With one PCR but two subsequent hybridizations the detection and differentiation of M. tuberculosis complex and 40 clinically relevant NTM species is possible.
  • Allows the detection of even weak-positive cultures and mixed cultures from fast and slow-growing mycabacteria.
  • Results are obtained within 5 hrs compared to several weeks with conventional methods.

Mycobacterium Species Detected:

  1. GenoType Mycobacterium CM
    • M. tuberculosis complex
    • and 24 of the most common NTM species
  2. GenoType Mycobacterium AS
    • permits the simultaneous molecular genetic identification of 19 additional NTM species.

A study published in US National Library of Medicine National Institute of Health done in Greece used these kits to analyzed 76 Nontuberculous Mycobacterial isolates. GenoType correctly identified all but one of the mycobacterial species. See full article here.

Genotype Mycobacterium CM/AS Interpretation

Methodology:

The GenoTypeMycobacterium CM(Common Mycobacteria) / AS (Additional Species) test is based on the DNA•STRIP technology and permits the identification of the following mycobacterial species: M. avium ssp., M. chelonae, M. abscessus, M. fortuitum, M.gordonae, M. intracellulare, M. scrofulaceum, M. interjectum, M. kansasii, M. malmoense, M. peregrinum, M. marinum/M. ulcerans, the M. tuberculosiscomplex and M. xenopi. The whole procedure is divided into three steps: DNA extraction from cultured material (culture plates/liquid medium; the necessary reagents are not provided), a multiplex amplification with biotinylated primers (the necessary thermostable DNA polymerase is not provided), anda reverse hybridization. The hybridization includes the following steps: chemical denaturation of the amplification products, hybridization of the single-stranded, biotin-labeled amplicons to membrane-bound probes, stringent washing, addition of a streptavidin/alkaline phosphatase (AP) conjugate, and an AP mediated staining reaction. A template ensures the easy and fast interpretation of the banding pattern obtained.

See Below for Full Documentations…

Genotype Mycobacterium AS

Genotype Mycobacterium CM